DeepVime (1): In Vivo Efficacy Profiling of Drugs and Foods Based on Saliva Samples

DeepVime: In Vivo Efficacy Profiling of Drugs and Foods Based on Saliva Samples (1) — Nutrient, Signaling Pathway, and Natural Product Pathway Changes

Industry Pain Points

When developing traditional Chinese medicines, cell therapies, and natural nutritional products, we face two major challenges: the complexity of product composition and the complexity of human responses. These are like facing two opaque "black boxes":

- In cell therapy: Traditional methods require months to observe treatment effects, lacking detection methods that can identify bodily responses early, making it difficult to predict treatment outcomes in advance

- In traditional Chinese medicine: The same treatment produces vastly different effects across individuals, and there is still a lack of scientific data analysis methods to explain these differences and mechanisms of action

Technological Breakthrough

DeepVime (Deep Visualization of In-vivo Mechanism) is an in vivo efficacy profiling technology that detects and analyzes the efficacy and mechanisms of various therapies by comparing changes in DNA methylation information in human saliva before and after health interventions. This technology achieves daily monitoring precision and is applicable to multiple therapeutic modalities, including pharmaceuticals, cell therapy, physical therapy, lifestyle interventions, exercise therapy, and food-drug therapy.

DeepVime is developed by DeepOMe (深度甲基), based on its proprietary human aging large model, the Capome multi-dimensional aging detection system, and an exclusive saliva DNA methylation real-world dataset.

Unique Advantages

- Individualized efficacy monitoring: Only requires two saliva samples — before and after intervention

- Signaling pathway tracking: Capable of identifying changes in in vivo signaling pathways, such as significant alterations in mitochondrial function

- Gene network analysis: Can track changes in gene networks, such as discovering changes in the NGFR (nerve growth factor receptor) gene network to understand in vivo targets and mechanisms of anxiety-relief and sleep-promoting products

Core Value

- Scientific evidence-based: Resolving the dilemma of traditional therapies being "effective but difficult to explain," providing mechanistic-level scientific evidence for TCM formulas, cell therapies, and functional foods

- Accelerated product development: Enabling rational design, precise combination, and targeted optimization through multi-dimensional profiling, transforming the R&D model from "trial-and-error development" to "data-driven iteration"

- Marketing breakthrough: Creating a "mechanism-defined" brand label, achieving precise population targeting and personalized matching, enhancing consumer scientific awareness and repurchase rates

- AI drug development: Establishing a complete data analysis system using real-world data, developing AI-based drug discovery systems

DeepVime In Vivo Efficacy Profiling Case Study

- Subject: Male, 48 years old

- Sample: Saliva, before and after consumption (day 0, days 1, 2, 3, and 4 after consumption)

- Intervention product: Two types of casein hydrolyzed peptides

- Reported symptoms: Reduced anxiety, more stable and pleasant mood after consumption; faster sleep onset, enhanced sensation of unobstructed breathing; refreshed upon waking, increased salivation

I. Post-Intervention Nutrient Pathway Changes

On day 2 after intervention, the ranked changes in nutrient-related biological processes are shown in the figure.

Under the casein hydrolyzed peptide product intervention, the "targeted efficacy index" (Action Score) of L-Tyrosine ranked highest among the substances shown in the figure, indicating that casein hydrolyzed peptides have the most significant impact on tyrosine-related biological processes. This may be related to the release of tyrosine upon casein hydrolysis, which then plays a key role in related physiological activities.

II. Post-Intervention Signaling Pathway Changes

On day 2 after intervention, the ranked changes in signaling pathways are shown in the figure.

These signaling pathways are correlated with anxiety and sleep regulation, specifically as follows:

- Nervous system development and structural maintenance: For example, GO POSITIVE REGULATION OF NERVOUS SYSTEM DEVELOPMENT directly affects neural development, GO DENDRITIC TREE and GO AXON relate to neuronal structure and affect neural signal transmission, which is crucial for the neural regulatory mechanisms of anxiety and sleep

- Signal transduction and cellular regulation: REACTOME DAG AND IP3 SIGNALING and REACTOME SIGNALING BY WNT participate in cellular signal transduction, affecting neurotransmitter release and neural plasticity, and neural plasticity abnormalities are closely related to anxiety and sleep disorders

- Immune and overall regulation: GO POSITIVE REGULATION OF IMMUNE SYSTEM PROCESS and related terms affect neural function through inflammatory responses, and chronic inflammation has been confirmed to be associated with the development of anxiety and insomnia

- Endocrine regulation: GO GLANDULAR EPITHELIAL CELL DIFFERENTIATION, if abnormal, may cause endocrine gland dysfunction, leading to dysregulation of thyroid hormones, cortisol, and other secretions, disrupting the neuro-endocrine axis and thereby affecting anxiety and sleep regulatory mechanisms

Overall, these biological processes affect neural, immune, and cellular regulation through multiple pathways, providing a multi-dimensional perspective for understanding the complex mechanisms of anxiety and sleep regulation, helping to further explore relevant physiological and pathological processes, and providing a theoretical foundation for intervention strategies.

III. Post-Intervention Natural Product Pathway Changes

On day 2 after intervention, the ranked changes in natural product-related pathways are shown in the figure.

These natural products exhibit certain common characteristics in health regulation:

- Neuroregulation-related: Some components involve neurotransmitter or neural pathway regulation. For example, INOSITOL participates in serotonin and other neurotransmitter signal transduction, with potential effects on anxiety regulation; OXYTOCIN can inhibit amygdala activity, alleviate anxiety, and promote deep sleep

- Anti-inflammatory and antioxidant: ARBUTIN, FUMARIC ACID, and others have antioxidant or anti-inflammatory properties, which can reduce the impact of oxidative stress and inflammation on neural function, indirectly maintaining nervous system health and supporting anxiety and sleep regulation

- Metabolic and physiological regulation: PROPIONIC ACID, VALERIC ACID, and others may participate in metabolic regulation; LINOLEIC ACID, as a fatty acid, participates in cell membrane composition, affecting neural signal transmission efficiency

If the above natural products (such as inositol, etc.) and casein hydrolyzed peptides act on related pathways (neuroregulation, endocrine, etc.), they can enhance effects through mechanistic complementarity, multi-target regulating anxiety, sleep, and other physiological processes. This provides direction for developing more effective products.

Follow-up articles: DeepVime: In Vivo Efficacy Profiling of Drugs and Foods Based on Saliva Samples (2) — TCM Syndrome Efficacy Changes

DeepVime: In Vivo Efficacy Profiling of Drugs and Foods Based on Saliva Samples (3) — Daily Dynamic Change Analysis